antibodies abs usedwere cdk4  (Cell Signaling Technology Inc)

Journal: Bioactive Materials

Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer

doi: 10.1016/j.bioactmat.2025.12.039

Figure Lengend Snippet: Metabolomic Analysis Reveals Mg and Al-Mg Induce Metabolic Reprogramming in Hepatocellular and Pancreatic Cancer Cells. (A) Metabolomic profiling of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups using LC-MS identified 1824 metabolites. (B) PCA illustrating clustering among different cell groups. (C) Heatmap showing differential abundances of characteristic metabolites across cell groups. (D) K-means clustering analysis highlighting metabolic differences among the cell groups. (E) Venn diagram displaying common differential metabolites among treatment groups. (F) Volcano plots of differential metabolites following Mg or Al-Mg treatment. (G) KEGG pathway enrichment analysis of differential metabolites. (H) Enrichment distribution of differential metabolites in Huh7 or PANC-1 cells treated with Mg or Al-Mg. (I) Quantitative analysis of intracellular metabolites including L-glutamine, adenine, uridine, cytidine, and guanine by ELISA with Mg or Al-Mg exposure. (J) Western blot analysis of p21, CDK4, and PCNA expression in PANC-1 cells after Mg or Al-Mg exposure. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), CPT1B (1:1000, Proteintech, 22170-1-AP), CDK4 (1:1000, Proteintech, 11026-1-AP), PCNA (1:1000, Proteintech, 10205-2-AP), p21 (1:1000, Proteintech, 10355-1-AP), GAPDH (1:1000, Proteintech, 60004-1-Ig) followed by HRP-conjugated secondary antibody (1:5000, Proteintech, RGAR001) for 1 h at room temperature.

Techniques: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Journal: Frontiers in Medicine

Article Title: Bushen Huoxue recipe restores trophoblast proliferation through the PI3K/AKT pathway in recurrent spontaneous abortion

doi: 10.3389/fmed.2026.1719434

Figure Lengend Snippet: Proliferation of placental tissues from mice. (A,B) Immunofluorescence of Ki67 and PCNA in placental tissues. (C,D) The protein expression of CyclinD1, CDK4, and CDK6 in placental tissues, detected by western blotting, n = 6; (E–G) Immunofluorescence of the indicated proteins. Scale bar = 20 μm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. the Con group; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. the RSA group. The data represent the mean ± SEM.

Article Snippet: Antibodies targeting CDK4 (R023806) and CK7 + (M012346) were purchased from Epizyme, China, Ltd. Antibodies against CDK6 (F0372) were supplied by Selleck, China, Ltd. Antibodies against Ki67 (ab16667) were purchased from Abcam (Shanghai, China).

Techniques: Immunofluorescence, Expressing, Western Blot